The overall interest of the lab
is the study of bacterial pathogens associated with Central Nervous System
disease. We examine the mechanisms by which
bacteria penetrate the blood-brain barrier, in order to cause meningitis. Our
studies focus on major human pathogens including Streptococcus, Staphylococcus
aureus and Bacillus anthracis. We seek to identify bacterial genes and gene
products responsible for cellular invasion, and to characterize the host innate
immune response to infection.
Lab
website
Selected Publications:
Banerjee, A., Kim, B. J., Carmona, E.M., Cutting, A.S., Gurney, M.A., Carlos, C., Feuer, R., Prasadarao, N.V, and Doran, K.S. 2011. Bacterial Pili exploit integrin machinery to promote immune activation and efficient blood-brain barrier penetration. Nature Communications. Sept 6, DOI: 10:1038/ncomms1474.
Ebrahimi, C.M., Sheen, T.R., Renken, C.W., Gottlieb, R.A., and Doran, K.S. 2011. Contribution of lethal toxin and edema toxin to the pathogenesis of anthrax meningitis. Infect. Immun. 79(7): 2510-2518.
van Sorge, N.M, Zialcita, P., Browne, S.H., Quach D, Guiney, D., and Doran, K.S. 2011. Penetration and activation of brain endothelium by Salmonella enterica serovar Typhimurium. J. Infect. Dis. 203(3): 401-405.
Banerjee, A., van Sorge, N.M., Sheen, T.R., Uchiyama S, Mitchell TJ, and Doran, K.S. 2010. Activation of brain endothelium by pneumococcal neuraminidase NanA promotes bacterial internalization. Cellular Microbiol. 12(11): 1576-1588.
Lembo, A., Gurney, M.A., Burnside, K., Banerjee, A., de Los Reyes, M., Connelly, J.E., Lin, W.J., Jewell, K.A., Vo, A., Renken, C.W., *Rajagopal. L., *Doran, K.S. (*co-corresponding authors). 2010. Regulation of CovR expression in Group B streptococcus impacts blood-brain barrier penetration. Mol. Microbiol. 77(2): 431-443.
Sheen, T.R., Ebrahimi, C.M., Heimstra, I.H., Barlow, S. B., Peschel, A., and Doran, K.S. 2010. Penetration of the blood-brain barrier by Staphylococcus aureus: Contribution of membrane-anchored lipoteichoic acid. J. Mol. Med. 88(6) 633-639.
Ebrahimi, C.M., Kern, J.W., Sheen, T.R., Ebrahimi-Fardooee, M.A., van Sorge, N.M., Schneewind, O., and Doran, K.S. 2009. Penetration of the blood-brain barrier by Bacillus anthracis requires the pXO1-encoded BslA protein. J. Bacteriol. Dec; 191(23): 7165-7173.
Uchiyama S, Carlin AF, Khosravi A, Weiman, S, Banerjee, A, Quach D, Hightower G, Mitchell TJ, Doran, K.S. and Nizet, V. 2009. The surface-anchored NanA protein promotes pneumococcal brain endothelial cell invasion. J. Exp. Med. 206 (9): 1845-1852.
van Sorge, N.M., Quach, D, Gurney, M.A., Sullam, P.M., Nizet V. and Doran, K.S. 2009. The group B streptococcal Srr-1 glycoprotein mediates penetration of the blood-brain barrier. J. Infect. Dis. 199 (10): 1479-1487. (featured on cover)
Quach, D, van Sorge, N.M., Kristin, S., Bryan, J., Shelver, D.W. and Doran, K.S. 2009. The CiaR response regulator in group B streptococcus promotes intracellular survival and resistance to innate immune defenses. J. Bacteriol. 191(7): 2023-32.
van Sorge, N.M.,
Quach, D, Gurney, M.A., Sullam, P.M., Nizet V. and Doran, K.S. 2009. The group B
streptococcal Srr-1 glycoprotein mediates penetration of the blood-brain
barrier. J. Infect. Dis. 199 (10): 1479-1487. (featured on cover)
Quach, D, van Sorge,
N.M., Kristin, S., Bryan, J., Shelver, D.W. and Doran, K.S. 2009. The CiaR response regulator in group B
streptococcus promotes intracellular survival and resistance to innate immune
defenses. J. Bacteriol. 191(7): 2023-32. (Epub 2008 Dec 29)
van Sorge, N.M., Ebrahimi, C., McGillivray, S., Quach, D, Sabet, M., Guiney, D., and Doran, K.S. 2008. Anthrax toxins inhibit neutrophil signaling pathways in brain endothelium and contribute to the pathogenesis of meningitis. PLoSONE. 3(8): 2964.
van Sorge, N.M.,
Ebrahimi, C., McGillivray, S., Quach, D, Sabet, M., Guiney, D., and Doran,
K.S. 2008. Anthrax toxins inhibit neutrophil signaling pathways in brain
endothelium and contribute to the pathogenesis of meningitis. PLoSONE.
3(8): 2964.
Maisey HC, Quach D,
Hensler ME, Liu GY, Nizet V. and Doran,
K.S. 2008. A group B streptococcal pilus protein promotes virulence and
phagocyte resistance. FASEB J. 6: 1715-1724.
Doran, K.S., Engelson, E.J.,
Khosravi, A., Maisey, H.C., Fedtke, I., Equils, O., Michelsen, K.S., Arditi,
M., Peschel, A., and Nizet, V. 2005. Blood-brain
barrier invasion by group B streptococcus depends upon proper cell-surface
anchoring of lipoteichoic acid. J. Clin. Invest. 115: 2499-2507.
Liu, G.Y., Doran, K.S., Lawrence, T., Turkson, N.,
Puliti, M., Tissi, L., Nizet, V. 2004. Sword and shield: linked group B streptococcal B-hemolysin/cytolysin
and carotenoid pigment act synergistically to subvert phagocytic defenses. Proc.
Natl. Acad. Sci. 101: 14491-14496.
Doran, K.S. and Nizet, V. 2004. Molecular pathogenesis of neonatal group
B streptococcal infection: no longer in its infancy. Mol. Microbiol. 54:
23-31.
Doran, K.S., Liu, G.Y., Nizet,
V. 2003. Group B streptococcal
B-hemolysin/cytolysin activates neutrophil signaling pathways in brain
endothelium and contributes to development of meningitis. J. Clin. Invest.
112: 736-744.